ColonyDoc-It™ Imaging Station Instruction Manual UVP, LLC Ultra-Violet Products Ltd. 2066 W 11th Street, Upland, CA 91786 Unit 1, Trinit
ColonyDoc-It Imaging Station 10 Installing the Filter Note: Filters come as an optional accessory so this step is only necessary if an optical fi
ColonyDoc-It Imaging Station 11 Installing the Software Insert the Doc-It Colony Counter CD into the CD drive in the computer. The InstallShield
ColonyDoc-It Imaging Station 12 Complete all required information on the form. Fill out the Serial Number located on the CD. The number should b
ColonyDoc-It Imaging Station 13 Counting Colonies Quick Capture Place the Petri dish to be counted on the transillumination surface. Select th
ColonyDoc-It Imaging Station 14 Insert the Petri dish inside the colony counter. Click the Preview button in the Camera Control module and move
ColonyDoc-It Imaging Station 15 Lighting Tips: There are four options for lighting the Petri dish. Epi Lighting options include White light and
ColonyDoc-It Imaging Station 16 The automated count will be displayed on the left hand side of the screen. Colonies can be filled in with red (as
ColonyDoc-It Imaging Station 17 Step 1: Select Classes The window that appears after choosing Manual Count is the Manual Count Colony Wizard.
ColonyDoc-It Imaging Station 18 Sometimes applying Background Correction to an image improves the count. To apply background correction click o
ColonyDoc-It Imaging Station 19 Once the first colony is selected, the new image in the Step 1 of 2: Select classes window will show a black back
ColonyDoc-It Imaging Station 2
ColonyDoc-It Imaging Station 20 Creating Templates Templates allow the user to set colony counting parameters so that plates with similar proper
ColonyDoc-It Imaging Station 21 If the shape and size sliders are changed to capture additional colonies, click Create template in the Templates
ColonyDoc-It Imaging Station 22 The new window will display the Total colonies counted. Fluorescent Colony Counting Ensure that the doo
ColonyDoc-It Imaging Station 23 Finally, choose to count the colonies automatically, manually, or with a template. Refer to the Automatic Coun
ColonyDoc-It Imaging Station 24 Zone Analysis Zone analysis provides users with zone sizing information useful for applications such as inhibiti
ColonyDoc-It Imaging Station 25 Step 1: Select classes The window that appears after choosing Manual Count is the Manual Count Colony Wizard.
ColonyDoc-It Imaging Station 26 The Select Classes tab allows users to select the number of classes and zones in the Petri dish sample. To anal
ColonyDoc-It Imaging Station 27 Step 2: Finish The software will automatically move to the second tab Step 2 of 2: Finish after Count is selected
ColonyDoc-It Imaging Station 28 Renumbering Colony/Zone Values To enable easier statistical reporting of each colony or zone, the values assigned
ColonyDoc-It Imaging Station 29 Spiral Plate Counting To perform a spiral plate count click onto the Spiral checkbox and then select Start Colony
ColonyDoc-It Imaging Station 3 END USER LICENSE AGREEMENT PLEASE READ THE FOLLOWING AGREEMENT CAREFULLY This Agreement is between UVP, LLC of 206
ColonyDoc-It Imaging Station 30 To view the resulting Data Table analysis for the spiral plate count, click onto the + sign (expanded report show
ColonyDoc-It Imaging Station 31 Editing Colonies Add Colonies Users may add colonies by performing the initial count (Automated Count, Manual Cou
ColonyDoc-It Imaging Station 32 To use a larger circle to highlight the colony, go to the Analysis Display Settings. A new window will open. Br
ColonyDoc-It Imaging Station 33 The colony circle (filled in or outlined) will now disappear. The Total colonies count will change to remove the
ColonyDoc-It Imaging Station 34 Draw lines through two or more colonies that are close together and treated as one. Use the pointer and, while h
ColonyDoc-It Imaging Station 35 Merging Colonies Users may merge colonies by performing the initial count (Automated Count, Manual Count, or User
ColonyDoc-It Imaging Station 36 Using the Software Changing Filter Settings To reduce user initial set-up time, filters were created to count “tr
ColonyDoc-It Imaging Station 37 Preferences Open the Preferences window from File > Preferences. This window allows users to set new defaults
ColonyDoc-It Imaging Station 38 To change the Circle Radius, click the drop down arrow and select from the numbers to change circle pixel radius.
ColonyDoc-It Imaging Station 39 The Logging preferences window allows the user to define the log file path and the log level. To change the Log
ColonyDoc-It Imaging Station 4 create warranties, and/or conditions, or in any way increase the scope of this limited warranty. Licensee assumes
ColonyDoc-It Imaging Station 40 Measure Length: Allows users to draw a line and measure the distance between any two points on the image, unit
ColonyDoc-It Imaging Station 41 Total area of the class on the sample (in pixels) Percentage of area of the class on the sample Mean are
ColonyDoc-It Imaging Station 42 In the second window, the Property is listed alongside the Area. Several numerical values are listed which inclu
ColonyDoc-It Imaging Station 43 The second tab shows Colonies. The third tab shows Statistics.
ColonyDoc-It Imaging Station 44 Supporting 21 CFR Part-11 Compliance Purpose US - Food and Drug Administration (US-FDA) created and released Part
ColonyDoc-It Imaging Station 45 Print an Audit Trail (Image History) Open the image for which an Audit Trail print copy is needed. Go to T
ColonyDoc-It Imaging Station 46 Replacing Switches Follow these steps to replace the light switches on the cabinet. Refer to replacement parts fo
ColonyDoc-It Imaging Station 47 If the window is still inactive, remove the USB cable from the USB port on the computer and then plug the USB cab
ColonyDoc-It Imaging Station 48 Technical Support UVP offers expert technical support on all of our products. If there are any questions about t
ColonyDoc-It Imaging Station 5 Table of Contents Introduction ...
ColonyDoc-It Imaging Station 6 Usage ...
ColonyDoc-It Imaging Station 7 Introduction This manual covers the installation and user instructions for the ColonyDoc-It Imaging Station. The
ColonyDoc-It Imaging Station 8 System Requirements Operating System: Windows XP Professional with Service Pack-2 (32 bit only), Vista (32 bi
ColonyDoc-It Imaging Station 9 Installing the ColonyDoc-It Installing the Hardware Set the ColonyDoc-It Imaging Station on a level surface. P
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