Uvp HybriCycler Instrukcja Użytkownika

HybriCycler Hybridization Oven Operating Instructions IMPORTANT: Please read these instructions before operating your UVP HybriCyclerTM syst

HC-3000 HybriCycler Hybridization Oven 10 Situation Response G+C = 45-55% follow normal protocol G+C < 45% Lower salt and temp

HC-3000 HybriCycler Hybridization Oven 11 Protocol 1: Random priming method for tagging DNA with fluorescein-lebeled nucleotide and others This

HC-3000 HybriCycler Hybridization Oven 12 12. 1 mL DNA polymerase 13. Mix gently and incubate at 37°C for 1 hour 14. Stop the reaction by adding 2

HC-3000 HybriCycler Hybridization Oven 13 5. Denature the probe by placing the samples in the boiling water bath and heating for 10 minutes. Imme

HC-3000 HybriCycler Hybridization Oven 14 6. Tape the blot to a cardboard backing. 7. Cover with plastic wrap to prevent the blots from sticking

HC-3000 HybriCycler Hybridization Oven 15 Product Warranty UVP’s quality HC-3000 HybriCycler System is guaranteed to be free of defects in materia

HC-3000 HybriCycler Hybridization Oven 2 Introduction The HybriCycler Hybridization Oven is designed as a compact alternative for hybridization r

HC-3000 HybriCycler Hybridization Oven 3 HybriCycler Hybridization Oven Hardware The HybriCycler Oven is comprised of: Hybridization Chamber Th

HC-3000 HybriCycler Hybridization Oven 4 Set-Up Instructions This equipment is not intended for interconnection with any other devices. Use of th

HC-3000 HybriCycler Hybridization Oven 5 Operation HybriCycler functions: • The “Power” switch turns the HybriCycler ON or OFF. • The “Motor

HC-3000 HybriCycler Hybridization Oven 6 3. Press the “Motor” switch to the ON position. Setting the Temperature Controls Whenever power is a

HC-3000 HybriCycler Hybridization Oven 7 Care and Cleaning The drawer-style spill tray is designed to contain any spills that leak from any of th

HC-3000 HybriCycler Hybridization Oven 8 Hybridization Techniques There are really two main steps to a hybridization reaction: hybridizing two str

HC-3000 HybriCycler Hybridization Oven 9 1: Concentration of Species Target: How much target molecule depends on the species you expect to find.