Uvp BioChemi System with EpiChemi II Darkroom Instrukcja Użytkownika

BioChemi Image Acquisition and Analysis System Installation and User Instructions BioImaging Systems

4. To remove the computer cover, remove the screws from the back of the case and slide the case off the computer. 5. Install the Capture Kit boar

4. Lenses The BioChemi system comes equipped with zoom and fixed lenses. Zoom lens features: Components of the zoom lens are as follows: 1. Top Ri

5. Mounting the Camera 1. To attach the camera bracket to the top of the EpiChemi II darkroom, place the black rubber gasket over the four screw h

5. Elevate 1/8” and screw the mounting bolts into the camera. Tighten, straighten, and secure. Make sure the zoom lens and the light sealing gasket a

6. Installing LabWorks and Capture Driver Computer The computer power cable should plug directly into a power strip or surge protector. Find and lo

17. Click Device Manager Tab 18. Look for ‘Other Devices’ or ‘Sound Video Game Controllers’ category 19. Double click them to open the lists 20. L

7. Printer and Security Dongle Unpack the Thermal Printer (if purchased) and place this alongside the computer. The printer is supplied with a separ

3. AC in Connector (power input) • Connect to a wall outlet using the AC power cord supplied The printer power cable should plug directly from the

Section 4 - BioChemi Capture A. Non-Fluorescent Samples (Protein Gels) Transmitted White Light • Coomassie Blue • Sliver Stain • SYPRO Red • SYP

4. Under the Integration tab, select No Integration (On-Chip Integration will be unavailable, Sequential Integration and Dynamic Integration should no

BIOCHEMI IMAGE ACQUISITION AND ANALYSIS SYSTEM INSTALLATION AND ACQUISITION MANUAL Table of Contents Section 1: Introduction ...

9. Check the Preview tab to make sure there is at least 55 Millisec entered. Numbers smaller than this will result in the inability to see a live prev

B. Fluorescent Gels (DNA/RNA Gels/Some Protein) Transmitted UV Light • Ethidium Bromide • SYBR Green • SYBR Gold • Fluorescein • Rhodamine •

4. From the Preview tab, click on the Start Preview button. Preview Tab Start Preview button WWW.UVP.COM US Technical Support: 1.800.452.6

5. Click the Integration tab and make sure that No Integration, Sequential Integration and Dynamic Integration are unchecked. Integration Tab 5.

6. If you want to preview and acquire for the same exposure, the Lock Exposure Times needs to be checked in the Acquire menu. 7. Place the UVP targe

C. Chemiluminescent samples (Films and Membranes/Plates) Emitted light in a light tight environment. • The time to capture chemiluminescence with

10. Click on the Options button and the following options will display. 11. Depending on your sample and your experience with it, you have three m

No Integration – straight accumulation of signal on the chip. The image captured is representative of all of the information on the chip at the end of

Sequential Integration – Sequence of images captured from multiple on-chip integrations of increasing duration. Can be played back as a Digital Video

Regular Intervals– Enter total amount of time and total number of frames you want to capture. This will produce X frames of T/X seconds each (T = Tota

Section 1 - Introduction Congratulations on your recent BioChemi purchase! You have selected the most comprehensive BioImaging System available on t

Irregular Intervals – Select from the menu or create your own by entering in irregular intervals. Resulting frames will play back in sequence in “real

Dynamic Integration Auto Exposure – Automatic accumulated exposure of multiple on-chip integrations before image saturation. Application: Immunoblot

Dynamic Integration Total Time Exposure – Accumulated exposure of pre-set, on-chip integrations over a specified time interval with automatic Digital

stacked when played back through the Digital Video Playback mode. 4. Click Capture. This automatically captures any live image before the brightest p

Section 5: Technical Support Before you contact UVP’s Technical Support for further assistance, please be prepared to provide the following informati

Appendix A – Recommended Stains Filter Emission Ethidium Bromide Ethidium Bromide Coomassie Blue SYPRO Orange Coo

Appendix B – Troubleshooting Guide Cannot capture Image. When selecting the Camera icon on the toolbar, there is a tab named Signal tab, which has a

Appendix C – Maintenance Changing Bulbs in the EpiChemi II: Remove the Transilluminator to prevent any scratching to the glass surface. Tilt t

Remove bulbs by rotating the bulbs, approximately a ¼ turn, and pulling at the same time, much like replacing fluorescent overhead bulbs. Then replac

4. EpiChemi II Darkroom • Overhead 254 nm UV, 365 nm UV, and fluorescent white light • Enables the user to view the setup of the experiment with

Section 2 - Safety Information NOTE: All UVP High Performance Transilluminators are powerful sources of Ultraviolet (UV) radiation that can cause da

Section 3 - BioChemi Installation 1. EpiChemi II Darkroom Components of the EpiChemi II Darkroom are described here. Please refer to your packing

1. Unpack the EpiChemi II Darkroom and Benchtop transilluminator from the packaging materials. 2. Assemble the two together by placing the transill

2. BioChemi Cooled CCD Assembly and Installation 1. The camera AC power adapter should be plugged to the wall with the power cable provided. 2.

3. PCI Image Acquisition Board Installation Note: The Capture Board is relatively easy to install. If, however, you have reservations about install